lyse 测验

Some phages lyse their hosts using a holin-endolysin system; however, in others, there is no interaction between the holin and the endolysin genes in host lysis [ 47 ].

Clustering was performed using the CFX manager software by Bio-Rad. Total Protein Extracts and protein quantification In order to obtain protein extracts for Western blot analysis mESC were lysed with 100μl of RIPA buffer (Sigma-Aldrich) supplemented with 2mM of phenylmethylsulphonyl fluoride-PMSF (Sigma-Aldrich) and 2x Halt phosphatase inhibitor cocktail (Pierce, Rockford, IL) as described elsewhere [8 ].